FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2358762011> ?p ?o ?g. }

• W2358762011 endingPage "105" @default.
• W2358762011 startingPage "101" @default.
• W2358762011 abstract "OBJECTIVE To study the prokaryotic expression of extracellular ligand binding domains of chick tie-2, the purification, refolding conditions of the recombinant protein, and its anti-angiogeneic effect. METHODS A DNA fragment encoding extracellular ligand binding domains of chick tie-2 was obtained by PCR amplification using a previous constructed plasmid as a template. The amplified fragment was then inserted into prokaryotic expression vector pQE30, and was expressed in E.Coli XL-1 blue by adding isopropyl-beta-D-thiogalactoside(IPTG). The recombinant protein in inclusion bodies was purified by nickel-nitrilotriacetic acid (Ni-NTA) affinity chromatography under denatured conditions. Then the refolding of the purified protein was performed with gradient dialysis. The target protein was injected s.c. into mouse, and the antibody was detected by ELISA and Western blot analysis. The antibody was purified from the antiserum and then incubated with human umbilical endothelial vein cell (HUEVC) to find its anti-angiogenesis in vitro by using propidium iodide(PI) dying through FACS. Alginate encapsulated tumor cell assays were performed and micro-vessel density was determined by counting per high power field in the sections stained with an antibody reactive to CD31 to test its inhibition of angiogenesis. RESULTS The recombinant protein was highly expressed in E.Coli XL-1 blue, and the antibody produced in mouse could specifically recognize the recombinant protein. The purified antibody could induce apoptosis of HUEVC in vitro. The anti-angiogenic effect of the antibody could also be found in alginate-encapsulate tumor cell assay and by counting micro-vessel density. CONCLUSION The protein of extracellular ligand binding domains of chick tie-2 can be expressed at high level in the prokaryotic expression system, and the expressed protein can induce immune response in mouse. Furthermore, the antibody can induce the anti-angiogenic effect." @default.
• W2358762011 created "2016-06-24" @default.
• W2358762011 creator A5004624217 @default.
• W2358762011 creator A5007016148 @default.
• W2358762011 creator A5010770426 @default.
• W2358762011 creator A5016477165 @default.
• W2358762011 creator A5019355497 @default.
• W2358762011 creator A5024835172 @default.
• W2358762011 creator A5035528148 @default.
• W2358762011 creator A5036757924 @default.
• W2358762011 creator A5042175309 @default.
• W2358762011 creator A5043226608 @default.
• W2358762011 creator A5060068655 @default.
• W2358762011 creator A5063829918 @default.
• W2358762011 creator A5082772220 @default.
• W2358762011 date "2004-04-01" @default.
• W2358762011 modified "2023-09-28" @default.
• W2358762011 title "[Prokaryotic expression of extracellular ligand binding domains of chick tie-2 and its anti-angiogenesis effect]." @default.
• W2358762011 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15079788" @default.
• W2358762011 hasPublicationYear "2004" @default.
• W2358762011 type Work @default.
• W2358762011 sameAs 2358762011 @default.
• W2358762011 citedByCount "0" @default.
• W2358762011 crossrefType "journal-article" @default.
• W2358762011 hasAuthorship W2358762011A5004624217 @default.
• W2358762011 hasAuthorship W2358762011A5007016148 @default.
• W2358762011 hasAuthorship W2358762011A5010770426 @default.
• W2358762011 hasAuthorship W2358762011A5016477165 @default.
• W2358762011 hasAuthorship W2358762011A5019355497 @default.
• W2358762011 hasAuthorship W2358762011A5024835172 @default.
• W2358762011 hasAuthorship W2358762011A5035528148 @default.
• W2358762011 hasAuthorship W2358762011A5036757924 @default.
• W2358762011 hasAuthorship W2358762011A5042175309 @default.
• W2358762011 hasAuthorship W2358762011A5043226608 @default.
• W2358762011 hasAuthorship W2358762011A5060068655 @default.
• W2358762011 hasAuthorship W2358762011A5063829918 @default.
• W2358762011 hasAuthorship W2358762011A5082772220 @default.
• W2358762011 hasConcept C104317684 @default.
• W2358762011 hasConcept C123894998 @default.
• W2358762011 hasConcept C136071829 @default.
• W2358762011 hasConcept C153911025 @default.
• W2358762011 hasConcept C159654299 @default.
• W2358762011 hasConcept C179247698 @default.
• W2358762011 hasConcept C181199279 @default.
• W2358762011 hasConcept C185592680 @default.
• W2358762011 hasConcept C202751555 @default.
• W2358762011 hasConcept C203014093 @default.
• W2358762011 hasConcept C2776415932 @default.
• W2358762011 hasConcept C2777411675 @default.
• W2358762011 hasConcept C2777834122 @default.
• W2358762011 hasConcept C2780394083 @default.
• W2358762011 hasConcept C28406088 @default.
• W2358762011 hasConcept C40767141 @default.
• W2358762011 hasConcept C502942594 @default.
• W2358762011 hasConcept C55493867 @default.
• W2358762011 hasConcept C86803240 @default.
• W2358762011 hasConceptScore W2358762011C104317684 @default.
• W2358762011 hasConceptScore W2358762011C123894998 @default.
• W2358762011 hasConceptScore W2358762011C136071829 @default.
• W2358762011 hasConceptScore W2358762011C153911025 @default.
• W2358762011 hasConceptScore W2358762011C159654299 @default.
• W2358762011 hasConceptScore W2358762011C179247698 @default.
• W2358762011 hasConceptScore W2358762011C181199279 @default.
• W2358762011 hasConceptScore W2358762011C185592680 @default.
• W2358762011 hasConceptScore W2358762011C202751555 @default.
• W2358762011 hasConceptScore W2358762011C203014093 @default.
• W2358762011 hasConceptScore W2358762011C2776415932 @default.
• W2358762011 hasConceptScore W2358762011C2777411675 @default.
• W2358762011 hasConceptScore W2358762011C2777834122 @default.
• W2358762011 hasConceptScore W2358762011C2780394083 @default.
• W2358762011 hasConceptScore W2358762011C28406088 @default.
• W2358762011 hasConceptScore W2358762011C40767141 @default.
• W2358762011 hasConceptScore W2358762011C502942594 @default.
• W2358762011 hasConceptScore W2358762011C55493867 @default.
• W2358762011 hasConceptScore W2358762011C86803240 @default.
• W2358762011 hasIssue "2" @default.
• W2358762011 hasLocation W23587620111 @default.
• W2358762011 hasLocation W23587620112 @default.
• W2358762011 hasOpenAccess W2358762011 @default.
• W2358762011 hasPrimaryLocation W23587620111 @default.
• W2358762011 hasRelatedWork W2142553468 @default.
• W2358762011 hasRelatedWork W2348628812 @default.
• W2358762011 hasRelatedWork W2349517503 @default.
• W2358762011 hasRelatedWork W2352045827 @default.
• W2358762011 hasRelatedWork W2354435465 @default.
• W2358762011 hasRelatedWork W2355518944 @default.
• W2358762011 hasRelatedWork W2357426305 @default.
• W2358762011 hasRelatedWork W2357618199 @default.
• W2358762011 hasRelatedWork W2357675544 @default.
• W2358762011 hasRelatedWork W2361172396 @default.
• W2358762011 hasRelatedWork W2362988430 @default.
• W2358762011 hasRelatedWork W2369877836 @default.
• W2358762011 hasRelatedWork W2370284457 @default.
• W2358762011 hasRelatedWork W2381958483 @default.
• W2358762011 hasRelatedWork W2384042289 @default.
• W2358762011 hasRelatedWork W2397030661 @default.
• W2358762011 hasRelatedWork W2433705909 @default.
• W2358762011 hasRelatedWork W2979523113 @default.

• next