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- W2358771279 abstract "Objective To verify the existence and extent of cell apoptosis during folliclular atresia in the pig. Methods Follicles of different diameter were observed ultrastructurally,the granulosa cells were examined by both TUNEL and HE staining and the DNA of granulosa cells from the healthy and the atretic follicles were analyzed by agarose gel electrophoresis. Results There were more granulosa cells undergoing apoptosis in the atretic follicles than those in the healthy follicles in pig.The percentages of apoptotic granulosa cells revealed by TUNEL is 9% for the healthy follicles and 39 2% for the atretic follicles,and those revealed by HE staining is 3 6% for healthy follicles and 34% for atretic follicles,the percentages of apoptotic granulosa cells revealed by the two way were closely correlated with each other and the correlation coefficient is 0 94.Conclusion These results indicated that there is apoptosis during pig follicular atresia,and apoptosis occurred mainly in the granulosa layer.The percentages of apoptotic granulosa cells revealed by TUNEL and HE indicated that in some circumstances TUNEL may replace by HE staining to detect cell apoptosis." @default.
- W2358771279 created "2016-06-24" @default.
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- W2358771279 date "2002-01-01" @default.
- W2358771279 modified "2023-09-24" @default.
- W2358771279 title "DETECTION OF GRANULOSA CELL APOPTOSIS IN PORCINE ATRETIC FOLLICLES" @default.
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