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- W2358822012 abstract "Aim To construct a recombinant adenovirus vector pAd-NK4 with Gateway technology.Methods The NK4 gene was amplified by PCR with plasmid con-taining hepatocyte growth factor(HGF)/NK4 as tem-plate,and then cloned into adenovirus adshuttle vector pYr-adshuttle-6 to acquire recombinant plasmid pYr-adshuttle-6-NK4,from which,the expression cassette of NK4-IRES-EGPF was transfered to pAD/BL-DEST with LR recombinant reaction to acquire recombinant adenovirus plasmid pAd-NK4-IRES-EGFP.The correct clone was linearized and transfected into 293A cells for packing and amplified adenovirus pAd-NK4 was obtained and identified.The titer was determined by TCID50.The transfection efficiency was observed under the fluorescent microscope.Results The recombinant adenovirus vector containing human NK4 gene was constructed successfully with the titer 6.3×1011 PFU·L-1.This vector was found to infect HEK293 cells efficiently.Conclusion The recombinant adenovirus expressing NK4 gene and green fluorescence can be successfully constructed with Gateway technology,which lays the foundation for further study." @default.
- W2358822012 created "2016-06-24" @default.
- W2358822012 creator A5033335916 @default.
- W2358822012 date "2011-01-01" @default.
- W2358822012 modified "2023-09-23" @default.
- W2358822012 title "Gateway technical supported construction of a recombinant adenovirus vector pAd-NK4" @default.
- W2358822012 hasPublicationYear "2011" @default.
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