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- W2358837645 abstract "Objective To construct new lentivirus interfering vectors target on DcR3,to produce virus to inhibit the expression of DcR3 in KYSE150 esophageal squamous cell line.Methods A miR30 like oligo was designed to target the coding region of DcR3 gene.After PCR amplification,the product were digested with Ecor I and Xho I,and then inserted into the downstream of the EGFP coding sequence in p201 vector.The verified recombined vectors were co-transfect with pMD2g and psAX2 into 293FT packing cell line to produced the virus.The virus-containing cell culture supernatants were added into the KYSE150 cell culture.Finally the virus infected cells were enriched by FACS and the DcR3 expression level were evaluated by Real-time PCR and Western blotting.Results Three DcR3 targeted lentivirus interfering vector were generated.The virus were produced successfully and inhibited the expression level of DcR3 in different degree respectively.Conclusion The new miR30 likfe sequence based lentivirus interfering vector was successfully constructed,which can provide a basis for further study of DcR3 function." @default.
- W2358837645 created "2016-06-24" @default.
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- W2358837645 date "2009-01-01" @default.
- W2358837645 modified "2023-09-24" @default.
- W2358837645 title "Construction and identification of DcR3 targeted RNA interfering lentivirus vector based on a new miR30 like sequence" @default.
- W2358837645 hasPublicationYear "2009" @default.
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