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- W2358867284 abstract "Objective To observe the transfection efficiency of adenovirus vector mediated enhanced green fluorescent proteins(AV-EGFP) and lentivirus vector mediated enhanced green fluorescent proteins(LV-EGFP) in the isolated rabbit corneal stromal cells in vitro,and to investigate the feasibility of the better virus vector as one of the gene therapy vector for corneal disease.Methods The primary cultured and subcultured keratocytes were identified from New Zealand white rabbits and cells identification.The cells were exposed to different multiplicity of infection(MOI) of LV-EGFP and AV-EGFP in transfective groups and the same quality of blank culture medium in contrast group.We observed the expression of EGFP by inverted fluorescence microscope at 24 hours,48 hours and 72 hours and then calculated the transfection efficiency through flow cytometer.Results AV-EGFP transfected rabbit corneal stromal cells for 24 hours to 48 hours green fluorescence can be seen clearly from the beginning,and earlier than LV-EGFP(48 hours).MOI as or less than 1000,with the increase of MOI,cells transfection efficiency was gradually increase in LV-EGFP group,and there was statistical difference(all P0.05).MOI more than 10,cells transfection efficiency was gradually increased in AV-EGFP group,and there were statistical differences(all P0.05).At the same MOI(MOI1),the transfection efficiency of LV-EGFP was specially higher than AV-EGFP and the difference was statistically significant(all P0.05).Conclusions Adenovirus and the Lentivirus vector can be efficient in vitro transfecting rabbit corneal stromal cells,and Lentivirus vector transduction efficiency was higher than Adenovirus at the same MOI." @default.
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- W2358867284 date "2012-01-01" @default.
- W2358867284 modified "2023-10-15" @default.
- W2358867284 title "Efficiency comparison of gene transfer into the isolated rabbit corneal stoma cells in vitro using adenovirus and lentivirus vector" @default.
- W2358867284 hasPublicationYear "2012" @default.
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