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- W2358951428 abstract "To explore the biological functions of human B7-H3, a novel member of B7 superfamily, and to prepare its monoclonal antibody, the gene encoding the human B7-H3 protein was obtained by PCR. Digested with EcoR I and BamH I, the PCR product was cloned into corresponding region of pGEZ-Term vector. The recombinant plasmid together with its two helper virus vectors was cotranstected into the package cell 293T with LipfectAMINE 2000. Then the supernatant of 293T was used to infect L929 cells. Selected by zeocin after transfection, a stable cell line B7-H3/L929 expressing the human B7-H3 was established. The expression stability and efficiency of the target molecule was identified by RT-PCR, Western blotting and flow cytometry analysis. In the presence of anti-CD3 mAb, B7-H3/L929 was evidenced to have a potency to inhibit activated T cell proliferation and IFN-γ, IL-10 secrefion in vitro, but the inhibitory effect could be reversed by the signal of CD28. In conclusion, B7-H3 has a down-regulatory characteristic to activated T cells in vitro." @default.
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- W2358951428 date "2004-01-01" @default.
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- W2358951428 title "Construction of Human B7-H3 Transfected Cell Line and the Investigation of Its Bioactivity in vitro" @default.
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