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- W2359014760 abstract "AIM To evaluate the presence of tumor DNA in plasma from malignant patients and establish a new approach to detect gene mutation in plasma DNA. METHODS DNA extraction from plasma of 40 malignant patients by using Qiagen Blood Kit and the target DNA fragments were amplified by PCR, the production was analyzed by denaturing high performance liquid chromatography (DHPLC), and the mutation status obtained by DHPLC was proved by DNA sequencing. RESULTS The target fragments were obtained from all samples of 40 malignant patients and normal tissue DNA, 8 mutations were detected by DHPLC and confirmed by DNA sequencing from malignant patients, no mutation was found in examples that no mutation detected by DHPLC. CONCLUSION Plasma DNA can be amplified in malignant patients, DHPLC is an accurate, rapid and economic way to screen mutation in plasma DNA amplified fragments and plasma p53 gene mutation detection may be useful for cancer alerting, early diagnosis or prognosis prediction." @default.
- W2359014760 created "2016-06-24" @default.
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- W2359014760 date "2002-01-01" @default.
- W2359014760 modified "2023-09-25" @default.
- W2359014760 title "DHPLC detection of p53 gene mutation in plasma DNA from 40 malignant patients" @default.
- W2359014760 hasPublicationYear "2002" @default.
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