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- W2359043503 abstract "Objective To express recombinant multimer peptides from Cryptococcus Antigens in E.coli.Methods The DNA sequence encoding dimer,tetramer and octamer of peptide GMFDGLSGV derived from Cryptococcus Antigens were synthesized respectively,linked with PADRE,Trojan peptide and furin-sensitive linker RVKR.Then they were amplified by polymerase chain reaction(PCR),inserted into the expression vector pGEX-4T-1 and transformed into E.Coli BL21(DE3).After induced by isopropylthio-β-D-galactoside(IPTG),the recombinant protein was confirmed by SDS-PAGE and western-blot.Results The recombinant 32 000,36 000 and 42 000 multimer peptides protein were expressed and confirmed by SDS-PAGE and Western-blot.Conclusion The recombinant multimer peptides may provide the basis for developing novel Cryptococcus vaccine." @default.
- W2359043503 created "2016-06-24" @default.
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- W2359043503 date "2008-01-01" @default.
- W2359043503 modified "2023-09-23" @default.
- W2359043503 title "Recombinant Expression and Identification of the Multimer Peptide Derived from Cryptococcus Antigens" @default.
- W2359043503 hasPublicationYear "2008" @default.
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