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- W2359046304 abstract "Extracted the RNA from Ascaris suum and reverse-transcribed into cDNA.Ran PCR amplification and linked to PMD18 T vector,then transformed into E.coli Top10 and went on sequencing.cloned the gene into pET28a expression vector and made Restriction enzymes digestion analysis.The sequencing showed the cloned Cecropin 2,Cecropin 3,Cecropin 4 genes from Ascaris suum all contain 225 bp encoding an open reading frame of 74 amino acids with the deduced molecular weight around 8 kD and the isoelectric point 9.86、10.16、9.16 respectively.homology analysis indicated All 3 genes are of high homology with the sequences of corresponding Cecropin p genes registered on NCBI,and the differentiation of Cecropin p4 preceded that of the others showed by phylogenic tree.The genes of Cecropin2,Cecropin3 and Cecropin4 were cloned successfully,expression vectors were constructed and the sequence analysis including Homological analysis,phylogenic tree were done,all of these has paved the way for the protein expression and the test of antimicrobial activities." @default.
- W2359046304 created "2016-06-24" @default.
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- W2359046304 date "2012-01-01" @default.
- W2359046304 modified "2023-09-26" @default.
- W2359046304 title "The Cloning and the Construction of Expression Vector of Cecropin p from Ascaris Suum" @default.
- W2359046304 hasPublicationYear "2012" @default.
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