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- W2359060233 abstract "AIM: To observe the adhesion and growth of rat bone marrow mesenehymal stem ceils(rBMSCs) seeded on demineralized dentin matrix(DDM) in vitro and the odontogenic induction of DDM.METHODS: BMSCs were successfully isolated and cultured.The compatibility of the biomaterial was evaluated by MTT assay.The odontogenic differentiation of BMSCs induced by DDM was measured using Alizarin red staining,the alkaline phosphatase(ALP) activity and reverse transcription-polymerase chain reaction(RT-PCR) assay.RESULTS: The proliferation of BMSCs in DDM groups was obviously higher than that in the control groups.The higher levels of ALP activity and larger mineralizing nodes were also detected in the experiment groups.RT-PCR demonstrated mRNA expression of dentin sialophosphoprotein(DSPP) and dentin matrix protein1(DMP-1) in BMSCs induced by DDM.CONCLUSION: DDM possesses a good cellular compatibility with BMSCs.It can induce the odontogenic differentiation of BMSCs." @default.
- W2359060233 created "2016-06-24" @default.
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- W2359060233 date "2011-01-01" @default.
- W2359060233 modified "2023-09-28" @default.
- W2359060233 title "Effects of demineralized dentin matrix on the odontogenic differentiation potential of bone marrow mesenchymal stem cells in vitro" @default.
- W2359060233 hasPublicationYear "2011" @default.
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