Matches in SemOpenAlex for { <https://semopenalex.org/work/W2359077265> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2359077265 abstract "The objective of this study was to prepare monoclonal antibody against glycoprotein C(gC)protein of infectious bovine rhinotracheitis virus(IBRV)and to analyze its biological characteristic.BALB/c mice were immunized with purified recombinant gC protein expressed by E.coli and the spleen cells of immunized mouse were fused with SP2/0myeloma cells.An indirect ELISA using the purified gC protein was developed to screen positive antibody-producing cells.A hybridoma stably secreting MAb designated as 3D6was obtained against gC protein.The monoclonal antibody belongs to IgG1,and the light chain wasκ.The titers of supernatant and ascites were 6.4×103 and 1.28×105 as detected by ELISA,respectively.The result of western blot assay and IFA suggested that the MAb could recognize IBRV.A sandwich ELISA was established by using 3D6secreted monoclonal antibody.The results indicated that the ELISA possessed good specificity and higher sensitivity.The monoclonal antibody could be used to diagnose IBRV and further research gC protein function." @default.
- W2359077265 created "2016-06-24" @default.
- W2359077265 creator A5030676667 @default.
- W2359077265 date "2013-01-01" @default.
- W2359077265 modified "2023-09-23" @default.
- W2359077265 title "Development and Preliminary Application of Monoclonal Antibody against IBRV gC and Identification of Its Biological Characteristic" @default.
- W2359077265 hasPublicationYear "2013" @default.
- W2359077265 type Work @default.
- W2359077265 sameAs 2359077265 @default.
- W2359077265 citedByCount "0" @default.
- W2359077265 crossrefType "journal-article" @default.
- W2359077265 hasAuthorship W2359077265A5030676667 @default.
- W2359077265 hasConcept C104317684 @default.
- W2359077265 hasConcept C108625454 @default.
- W2359077265 hasConcept C153911025 @default.
- W2359077265 hasConcept C159047783 @default.
- W2359077265 hasConcept C159654299 @default.
- W2359077265 hasConcept C163864487 @default.
- W2359077265 hasConcept C203014093 @default.
- W2359077265 hasConcept C2776415932 @default.
- W2359077265 hasConcept C2780931953 @default.
- W2359077265 hasConcept C32611913 @default.
- W2359077265 hasConcept C36394416 @default.
- W2359077265 hasConcept C40767141 @default.
- W2359077265 hasConcept C542903549 @default.
- W2359077265 hasConcept C55493867 @default.
- W2359077265 hasConcept C86803240 @default.
- W2359077265 hasConceptScore W2359077265C104317684 @default.
- W2359077265 hasConceptScore W2359077265C108625454 @default.
- W2359077265 hasConceptScore W2359077265C153911025 @default.
- W2359077265 hasConceptScore W2359077265C159047783 @default.
- W2359077265 hasConceptScore W2359077265C159654299 @default.
- W2359077265 hasConceptScore W2359077265C163864487 @default.
- W2359077265 hasConceptScore W2359077265C203014093 @default.
- W2359077265 hasConceptScore W2359077265C2776415932 @default.
- W2359077265 hasConceptScore W2359077265C2780931953 @default.
- W2359077265 hasConceptScore W2359077265C32611913 @default.
- W2359077265 hasConceptScore W2359077265C36394416 @default.
- W2359077265 hasConceptScore W2359077265C40767141 @default.
- W2359077265 hasConceptScore W2359077265C542903549 @default.
- W2359077265 hasConceptScore W2359077265C55493867 @default.
- W2359077265 hasConceptScore W2359077265C86803240 @default.
- W2359077265 hasLocation W23590772651 @default.
- W2359077265 hasOpenAccess W2359077265 @default.
- W2359077265 hasPrimaryLocation W23590772651 @default.
- W2359077265 hasRelatedWork W1986246254 @default.
- W2359077265 hasRelatedWork W2106512775 @default.
- W2359077265 hasRelatedWork W2348466398 @default.
- W2359077265 hasRelatedWork W2355934132 @default.
- W2359077265 hasRelatedWork W2356338171 @default.
- W2359077265 hasRelatedWork W2361134912 @default.
- W2359077265 hasRelatedWork W2368460920 @default.
- W2359077265 hasRelatedWork W2368688748 @default.
- W2359077265 hasRelatedWork W2370715180 @default.
- W2359077265 hasRelatedWork W2371121527 @default.
- W2359077265 hasRelatedWork W2375823741 @default.
- W2359077265 hasRelatedWork W2387738693 @default.
- W2359077265 hasRelatedWork W2388873006 @default.
- W2359077265 hasRelatedWork W2391273643 @default.
- W2359077265 hasRelatedWork W2392279689 @default.
- W2359077265 hasRelatedWork W2413889891 @default.
- W2359077265 hasRelatedWork W2435920612 @default.
- W2359077265 hasRelatedWork W2617075535 @default.
- W2359077265 hasRelatedWork W2978251081 @default.
- W2359077265 hasRelatedWork W1929147813 @default.
- W2359077265 isParatext "false" @default.
- W2359077265 isRetracted "false" @default.
- W2359077265 magId "2359077265" @default.
- W2359077265 workType "article" @default.