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- W2359080991 abstract "OBJECTIVE:To explore the proliferation capacity and phenotypic characteristics of CIK cells induced from cryo-preserved peripheral blood progenitor cells(PBPCs) of patients and the fresh PBMCs of healthy donors,and compare their cytotoxicities against B lymphoma cell line in vitro.METHODS:Mononuclear cells were isolated from cryo-preserved PBPCs of the patients and fresh peripheral blood of health donors by Ficoll-hypaque density centrifugation,and then induced by IFNγ,IL-2 and CD3mAb.The proliferation capacity of CIK cells was detected by cell recording board;the phenotypes of CIK cells were analyzed by FACS;target cells were differentiated from effect cells by CFSE dying.The mortality of target cells was determined by FACS.RESULTS:The CIK cells induced from cryo-preserved PBPCs of the patients proliferated much more slowly than those from the the fresh PBMCs of healthy donors,and its maximum proliferation multiple was also lower.But the proportion of CD3+ CD56+ cell showed no significant differences between them,being (16.14±8.49)% and (17.52±3.30)% respectively (P=0.744).The cytotoxicity of patients and health donors’s CIK cells was significantly stronger than that of the uncultured PBMCs (P were 0.004 and 0.001).With the effector-target ratio 10:1,the activities against daudi cell line of the CIK cells of the two groups were (29.23±8.85)% and (20.13±2.36)%,and the activities of patients’s CIK cells were more stronger than those of the health donors (P=0.05).CONCLUSIONS:CIK cells owning strong anti-tumor ability can be induced from the cryo-preserved PBPCs of patients.Our findings provide an experimental basis for new applications of cryo-preserved PBPCs in clinic." @default.
- W2359080991 created "2016-06-24" @default.
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- W2359080991 date "2010-01-01" @default.
- W2359080991 modified "2023-09-24" @default.
- W2359080991 title "Expansion of CIK cells from cryopreserved peripheral blood progenitor cells and their anti-tumor effect in vitro" @default.
- W2359080991 hasPublicationYear "2010" @default.
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