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- W2359101065 abstract "The best concentration of IPTG,the temperature and time of inducement of the recombinant plasmids σNS-pGEX-4T-1 were explored.The result showed that the express fusion protein is of a molecular weight of 66 200,amounts to 31.5%-33.3% of the total bacterial protein.The best concentration of IPTG is 0.8 mmol/L,the temperature is 28-37℃,and the time is 4 h.Most of the fusion protein is insoluble when induced in 37℃ and 32℃,and is soluble in 28℃.The σNS protein is induced in 28℃ and purified by Sepharose 4B.The concentration of σNS protein after purification is 0.91 g/L,amounting to 93% of the total protein.Western-blot analysis with ARV antibodies against the purified fusion protein reveals a good antigenicity." @default.
- W2359101065 created "2016-06-24" @default.
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- W2359101065 date "2008-01-01" @default.
- W2359101065 modified "2023-09-27" @default.
- W2359101065 title "Prokaryotic expression and purification of σNS protein of avian reovirus" @default.
- W2359101065 hasPublicationYear "2008" @default.
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