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- W2359105013 abstract "Objective:Protoplasts of Populus alba L.var.pyramidalis were isolated from calli,isolation and purification of protoplasts were studied.Methods:Using simple experimental design and variance analysis method,according to the influence factors of protoplasts isolation,isolated time and mannitol concentration of protoplasts were studied,by using FDA staining method observing viability of calli protoplasts.Results:The more appropriate yield of protoplasts was produced by using CPW salts solution containing 2.0% cellulase R-10,1.0% macerozyme R-10,1.0% pectolase Y-23,0.6 mol/L mannitol which incubated at 27℃ for 8 hours.The protoplasts yield was 8.5×106/(g·FW) and the viability was 83.6% under the condition.Moreover,the more suitable protoplasts purification method was sucrose gradient centrifugation at concentration of 30%.Conclusion:The combination of different enzyme concentrations and factors is appropriate conducted on investigation into isolation and purification of calli-derived protoplasts." @default.
- W2359105013 created "2016-06-24" @default.
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- W2359105013 date "2011-01-01" @default.
- W2359105013 modified "2023-09-27" @default.
- W2359105013 title "Isolation and Purification of Callus-Derived Protoplasts of Populus alba L.var. pyramidalis" @default.
- W2359105013 hasPublicationYear "2011" @default.
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