Matches in SemOpenAlex for { <https://semopenalex.org/work/W2359110332> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2359110332 abstract "Objective To achieve the prokaryotic expression of pQE-vctB(recombinant gene containing VacA cytotoxin segment from H.pylori and cholera toxin B subunit gene)in E.coli DH5 alpha to obtain recombinant protein,identify its antigenicity and immunogenicity,and lay the foundation for the preparation of oral vaccine for prevention of H.pyloriinfection.Methods(1)The pQE-vctB fusion gene was translated into E.coli DH5alpha;VCTB expression was induced by IPTG;Western blotting was used to analyze the antigenicity;nickel ion column was used for purification.(2)The mice were immunized with the recombinant protein of VCTB orally;ELISA was used to detecti the specific IgG in serum and IgA in intestinal washes of the immunized mice in order to identify the immunogenicity.Results SDS-PAGE showed the relative molecular weight of the recombinant VCTB protein was about 40 kD,and the purity was over 92% by affinity chromatography after purification.Western blotting showed it was able to react with VacA.ELISA assay showed that specific serum IgG and intestinal IgA were significantly higher than those of VacA control group(P0.01).Conclusion The recombinant protein VCTB was successfully expressed at high level and purity,and with satisfactory antigenicity and immunogenicity,which can be used for the preparation of oral vaccine for prevention of H.pylori infection." @default.
- W2359110332 created "2016-06-24" @default.
- W2359110332 creator A5050834238 @default.
- W2359110332 date "2015-01-01" @default.
- W2359110332 modified "2023-09-23" @default.
- W2359110332 title "Prokaryotic expression and immunological study of recombinant vacA-CtxB fusion protein of Helicobacter pylori" @default.
- W2359110332 hasPublicationYear "2015" @default.
- W2359110332 type Work @default.
- W2359110332 sameAs 2359110332 @default.
- W2359110332 citedByCount "0" @default.
- W2359110332 crossrefType "journal-article" @default.
- W2359110332 hasAuthorship W2359110332A5050834238 @default.
- W2359110332 hasConcept C104317684 @default.
- W2359110332 hasConcept C123894998 @default.
- W2359110332 hasConcept C147483822 @default.
- W2359110332 hasConcept C153911025 @default.
- W2359110332 hasConcept C159047783 @default.
- W2359110332 hasConcept C170344550 @default.
- W2359110332 hasConcept C179247698 @default.
- W2359110332 hasConcept C181199279 @default.
- W2359110332 hasConcept C185592680 @default.
- W2359110332 hasConcept C203014093 @default.
- W2359110332 hasConcept C2779026604 @default.
- W2359110332 hasConcept C2780868878 @default.
- W2359110332 hasConcept C40767141 @default.
- W2359110332 hasConcept C55493867 @default.
- W2359110332 hasConcept C85265743 @default.
- W2359110332 hasConcept C86803240 @default.
- W2359110332 hasConcept C89423630 @default.
- W2359110332 hasConceptScore W2359110332C104317684 @default.
- W2359110332 hasConceptScore W2359110332C123894998 @default.
- W2359110332 hasConceptScore W2359110332C147483822 @default.
- W2359110332 hasConceptScore W2359110332C153911025 @default.
- W2359110332 hasConceptScore W2359110332C159047783 @default.
- W2359110332 hasConceptScore W2359110332C170344550 @default.
- W2359110332 hasConceptScore W2359110332C179247698 @default.
- W2359110332 hasConceptScore W2359110332C181199279 @default.
- W2359110332 hasConceptScore W2359110332C185592680 @default.
- W2359110332 hasConceptScore W2359110332C203014093 @default.
- W2359110332 hasConceptScore W2359110332C2779026604 @default.
- W2359110332 hasConceptScore W2359110332C2780868878 @default.
- W2359110332 hasConceptScore W2359110332C40767141 @default.
- W2359110332 hasConceptScore W2359110332C55493867 @default.
- W2359110332 hasConceptScore W2359110332C85265743 @default.
- W2359110332 hasConceptScore W2359110332C86803240 @default.
- W2359110332 hasConceptScore W2359110332C89423630 @default.
- W2359110332 hasLocation W23591103321 @default.
- W2359110332 hasOpenAccess W2359110332 @default.
- W2359110332 hasPrimaryLocation W23591103321 @default.
- W2359110332 hasRelatedWork W2037754333 @default.
- W2359110332 hasRelatedWork W2189564038 @default.
- W2359110332 hasRelatedWork W2276385301 @default.
- W2359110332 hasRelatedWork W2290893319 @default.
- W2359110332 hasRelatedWork W2383787229 @default.
- W2359110332 hasRelatedWork W2402484903 @default.
- W2359110332 hasRelatedWork W2407779263 @default.
- W2359110332 hasRelatedWork W2408800467 @default.
- W2359110332 hasRelatedWork W2432717918 @default.
- W2359110332 hasRelatedWork W2463650759 @default.
- W2359110332 hasRelatedWork W2586433927 @default.
- W2359110332 hasRelatedWork W2792298935 @default.
- W2359110332 hasRelatedWork W2943907233 @default.
- W2359110332 hasRelatedWork W2944467783 @default.
- W2359110332 hasRelatedWork W2977873235 @default.
- W2359110332 hasRelatedWork W3013946072 @default.
- W2359110332 hasRelatedWork W3030912497 @default.
- W2359110332 hasRelatedWork W3032086174 @default.
- W2359110332 hasRelatedWork W3177627400 @default.
- W2359110332 hasRelatedWork W3184447697 @default.
- W2359110332 isParatext "false" @default.
- W2359110332 isRetracted "false" @default.
- W2359110332 magId "2359110332" @default.
- W2359110332 workType "article" @default.