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- W2359135231 abstract "Objective To investigate the conditions influencing the induction,differentiation,and rooting of callus in Herpetospermum pedunculosum,establish a new method for rapid propagation,and lay a foundation for plant tissue culture and regeneration.Methods With the young stems and leaves of H.pedunculosum as materials,effects of different explants,basic medium,and plant growth regulators on callus induction,differentiation,and rooting were compared.Results When young stems were used as explants,the induction rate was higher than that of leaves.MS medium could produce obvious callus with high induction rate.The optimum compositions for callus induction,differentiation,and rooting were MS+6-BA 2 mg/L+NAA 0.5 mg/L,MS+6-BA 2 mg/L+NAA 0.1 mg/L+KT 3.0 mg/L,and 1/2 MS+NAA 1.0 mg/L.The callus induction rate,differentiation rate,and rooting rate were 93.3%,87.2%,and 95%,respectively.Conclusion The young stems of H.pedunculosum could differentiate the adventitious buds in a suitable medium through callus,and be easy to take root.The regeneration plantlets with normal growth and development could be obtained." @default.
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- W2359135231 date "2012-01-01" @default.
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- W2359135231 title "Callus induction and plant regeneration in Herpetospermum pedunculosum" @default.
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