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- W2359156854 abstract "Objectives To observe biochemical changes of various plasma components during the methylene blue photochemical virus inactivation process and evaluate clinical application of virus inactived plasma.Methods A total of 200 virus inactivated blood samples,were randomly selected.The amount of plasma samples and the level of total protein,factor Ⅷ and fibrinogen were measued.We detected the concentration of methylene blue photochemistry.Plasma samples from 15 756 people,which have gotten negative results in HBsAg,anti-HCV and anti-HIV detecting with ELISA,were respectively detected the nucleic acid of HBV,HCV and HIV before and after virus inactivation.Furthermore,we randomly selected 200 patients who were transfused with virus inactivated plasma to invest ate if the adverse effects occurred more frequently after transfusion. Results After virus inactivating,the recovery rate of plasma volume,total protein,factor Ⅷ and fibrinogen were(96.67±2.34)%,(88.69±3.32) %,(86.84±2.16)% and(84.62±1.86)% respectively.There were no significant differences before and after virus inactivation(P0.05).Residual concentration of methylene blue was(0.16±0.03) μmol/L after virus inacivation and filtration.The remove rate was 86.32%.When plasma from 15 756 people were detected nucleic acid of HBV,HCV and HIV before virus inactivating,plasma from 23 people were found positive results of HBV DNA and plasma from 1 people was found positive result of HIV RNA.The positive rate was 1.52 per mill.But after virus inactivating,we got negative results when detecting nucleic acid of HBV,HCV and HIV.Meanwhile,the adverse effects were not found after transfusion of virus inactivated plasma.Conclusion Virus inactivation plasma prepared by methylene blue photochemistry is one of blood transfusion safety measures and when they were transfused to patients,adverse effects were not found." @default.
- W2359156854 created "2016-06-24" @default.
- W2359156854 creator A5032760911 @default.
- W2359156854 date "2013-01-01" @default.
- W2359156854 modified "2023-09-25" @default.
- W2359156854 title "Preparation of Virus Inactivea Plasma by Methylene Blue Photochemical Method and Evaluation of Its Effect on Clinical Application" @default.
- W2359156854 hasPublicationYear "2013" @default.
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