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- W2359203414 abstract "Aims To construct expression vector of CP23 on sporozoites of Cryptosporidium parvum and express it in E coli Methods A linear fragment of pET-28a(+) and CP23 gene of C parvum were obtained from the plasmids of pET-28a(+) and pMD18-T-23 with Hind Ⅲ and EcoRⅠdisgested The expression vector of CP23 was constructed by T 4 ligase and transformed into BL21(DE3) strain cells of E coli for expression The expression products were analyzed by SDS-PAGE,ELISA and Western blotting Results The expression vector of CP23 was constructed Distinct protein band with a molecular weight of 14 kDa was detected on SDS-PAGE and its antigenicity was confirmed by ELISA and Western blotting Conclusion Efficient expression of CP23 fusion protein has been achieved in E coli" @default.
- W2359203414 created "2016-06-24" @default.
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- W2359203414 date "2003-01-01" @default.
- W2359203414 modified "2023-09-24" @default.
- W2359203414 title "Expression of CP23 fusion protein on sporozoites of Cryptosporidium parvum in E.coli" @default.
- W2359203414 hasPublicationYear "2003" @default.
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