Matches in SemOpenAlex for { <https://semopenalex.org/work/W2359236422> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2359236422 abstract "Based on previous work, a novel gene fragment CG 14 related to spermatogenesis was cloned into the prokaryotic expression vector pGEX 3X. After restriction analysis,PCR identification and DNA sequencing, the positive clone was induced by IPTG. The expected protein was expressed in fusion form with a high yield. The expression product was purified by glutathione Sepharose 4B affinity chromatography and digested by factor Xa. Western blotting showed that the approximate molecular weight of the final product was 8 kD. Rabbits were immunized with the fusion protein, the specific antibody against the target protein was raised." @default.
- W2359236422 created "2016-06-24" @default.
- W2359236422 creator A5073749115 @default.
- W2359236422 date "2002-01-01" @default.
- W2359236422 modified "2023-09-25" @default.
- W2359236422 title "Cloning and Expression of a Novel Gene Fragment CG 14 Related to Spermatogenesis in E. coli" @default.
- W2359236422 hasPublicationYear "2002" @default.
- W2359236422 type Work @default.
- W2359236422 sameAs 2359236422 @default.
- W2359236422 citedByCount "0" @default.
- W2359236422 crossrefType "journal-article" @default.
- W2359236422 hasAuthorship W2359236422A5073749115 @default.
- W2359236422 hasConcept C104317684 @default.
- W2359236422 hasConcept C121050878 @default.
- W2359236422 hasConcept C123894998 @default.
- W2359236422 hasConcept C150194340 @default.
- W2359236422 hasConcept C153911025 @default.
- W2359236422 hasConcept C170344550 @default.
- W2359236422 hasConcept C179247698 @default.
- W2359236422 hasConcept C181199279 @default.
- W2359236422 hasConcept C19924922 @default.
- W2359236422 hasConcept C199360897 @default.
- W2359236422 hasConcept C33161422 @default.
- W2359236422 hasConcept C40767141 @default.
- W2359236422 hasConcept C41008148 @default.
- W2359236422 hasConcept C54355233 @default.
- W2359236422 hasConcept C55493867 @default.
- W2359236422 hasConcept C81089528 @default.
- W2359236422 hasConcept C85265743 @default.
- W2359236422 hasConcept C86803240 @default.
- W2359236422 hasConceptScore W2359236422C104317684 @default.
- W2359236422 hasConceptScore W2359236422C121050878 @default.
- W2359236422 hasConceptScore W2359236422C123894998 @default.
- W2359236422 hasConceptScore W2359236422C150194340 @default.
- W2359236422 hasConceptScore W2359236422C153911025 @default.
- W2359236422 hasConceptScore W2359236422C170344550 @default.
- W2359236422 hasConceptScore W2359236422C179247698 @default.
- W2359236422 hasConceptScore W2359236422C181199279 @default.
- W2359236422 hasConceptScore W2359236422C19924922 @default.
- W2359236422 hasConceptScore W2359236422C199360897 @default.
- W2359236422 hasConceptScore W2359236422C33161422 @default.
- W2359236422 hasConceptScore W2359236422C40767141 @default.
- W2359236422 hasConceptScore W2359236422C41008148 @default.
- W2359236422 hasConceptScore W2359236422C54355233 @default.
- W2359236422 hasConceptScore W2359236422C55493867 @default.
- W2359236422 hasConceptScore W2359236422C81089528 @default.
- W2359236422 hasConceptScore W2359236422C85265743 @default.
- W2359236422 hasConceptScore W2359236422C86803240 @default.
- W2359236422 hasLocation W23592364221 @default.
- W2359236422 hasOpenAccess W2359236422 @default.
- W2359236422 hasPrimaryLocation W23592364221 @default.
- W2359236422 hasRelatedWork W2326375097 @default.
- W2359236422 hasRelatedWork W2347270363 @default.
- W2359236422 hasRelatedWork W2347667885 @default.
- W2359236422 hasRelatedWork W2348255161 @default.
- W2359236422 hasRelatedWork W2349741016 @default.
- W2359236422 hasRelatedWork W2354608439 @default.
- W2359236422 hasRelatedWork W2356539089 @default.
- W2359236422 hasRelatedWork W2359596456 @default.
- W2359236422 hasRelatedWork W2364742102 @default.
- W2359236422 hasRelatedWork W2372039114 @default.
- W2359236422 hasRelatedWork W2375347875 @default.
- W2359236422 hasRelatedWork W2378592369 @default.
- W2359236422 hasRelatedWork W2381291606 @default.
- W2359236422 hasRelatedWork W2384571031 @default.
- W2359236422 hasRelatedWork W2386839470 @default.
- W2359236422 hasRelatedWork W2387935476 @default.
- W2359236422 hasRelatedWork W2388066713 @default.
- W2359236422 hasRelatedWork W2392852276 @default.
- W2359236422 hasRelatedWork W2392918163 @default.
- W2359236422 hasRelatedWork W2394260263 @default.
- W2359236422 isParatext "false" @default.
- W2359236422 isRetracted "false" @default.
- W2359236422 magId "2359236422" @default.
- W2359236422 workType "article" @default.