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- W2359288555 abstract "Objective To express human Y2 receptor protein in E.coli,purify and identify it,and conduct bioinformatic analysis of Y2 receptor protein.Methods The recombinant plasmid pET28a-Y2 which had been well constructed and sequentially confirmed was transplanted into E.coli BL21(DE3)and induced by IPTG to express fusion proteins.SDS-PAGE and Western blot were used to test and identify the expressed fusion proteins.The inclusion body of the expressed product was purified by Ni2+-NTA affinity chromatography.Then bioinformatic analysis of the Y2 receptor was conducted with the help of related online software.Results After being induced by IPTG,the DE3 with recombinant plasmid pET28a-Y2 expressed recombinant human Y2 receptor protein.Highly purified fusion protein was obtained by Ni2+-NTA affinity chromatography.Related biological characteristics of Y2 receptor were obtained after the online software analysis.Conclusions The recombinant plasmid pET28a-Y2 can be successfully expressed in DE3.Highly purified proteins can be obtained by Ni2+-NTA affinity chromatography.Y2 receptor's biological characteristics are predicted,which lays foundation for further studies of Y2 receptor protein's biological function and antibody development." @default.
- W2359288555 created "2016-06-24" @default.
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- W2359288555 date "2009-01-01" @default.
- W2359288555 modified "2023-09-25" @default.
- W2359288555 title "Study on expression,purification and bioinformatic analysis of recombinant human neuropeptideY Y2 receptor" @default.
- W2359288555 hasPublicationYear "2009" @default.
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