Matches in SemOpenAlex for { <https://semopenalex.org/work/W2359297762> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2359297762 abstract "Porcine circovirus type 2(PCV2) has been implicated as the etiological agent of a new disease,named postweaning multisystemic wasting syndrome(PMWS).PCV2 contains two major open reading frames(ORFs):ORF1 and ORF2,encoding replication-associated(Rep) protein and capsid(Cap) protein,respectively.In order to find out the relationship between structure and function of Rep protein,the ORF1 gene of PCV2 was cloned into baculovirus transfer vector(pFastBacTMDual) using Bac-to-Bac baculovirus expression system.E.coli DH10Bac containing baculovirus shutter vector(bacmid) and helper vector was transformed with recombinant plasmid pFBD-ORF1.The colonies of E.coli containing recombinant bacmid(rBacmid-ORF1) were collected by blue/white selection.The rBacmid-ORF1 was transfected into sf9 cells to yield Ac.NPV carrying the PCV2 ORF1 gene,referred to as rAc-Rep.Expression of the ORF1 gene of PCV2 was confirmed by SDS-PAGE and Western-blotting.The Rep protein expressed by rAc-Rep had a molecular weight of 36 ku." @default.
- W2359297762 created "2016-06-24" @default.
- W2359297762 creator A5062197376 @default.
- W2359297762 date "2007-01-01" @default.
- W2359297762 modified "2023-09-24" @default.
- W2359297762 title "Construction and identification of recombinant insect baculovirus expressing Rep protein of Porcine Circovirus type 2" @default.
- W2359297762 hasPublicationYear "2007" @default.
- W2359297762 type Work @default.
- W2359297762 sameAs 2359297762 @default.
- W2359297762 citedByCount "0" @default.
- W2359297762 crossrefType "journal-article" @default.
- W2359297762 hasAuthorship W2359297762A5062197376 @default.
- W2359297762 hasConcept C103878061 @default.
- W2359297762 hasConcept C104317684 @default.
- W2359297762 hasConcept C145640855 @default.
- W2359297762 hasConcept C153911025 @default.
- W2359297762 hasConcept C159047783 @default.
- W2359297762 hasConcept C167625842 @default.
- W2359297762 hasConcept C202878990 @default.
- W2359297762 hasConcept C22744801 @default.
- W2359297762 hasConcept C2522874641 @default.
- W2359297762 hasConcept C2777773057 @default.
- W2359297762 hasConcept C2780530800 @default.
- W2359297762 hasConcept C2781045504 @default.
- W2359297762 hasConcept C33161422 @default.
- W2359297762 hasConcept C40767141 @default.
- W2359297762 hasConcept C47289529 @default.
- W2359297762 hasConcept C54355233 @default.
- W2359297762 hasConcept C86803240 @default.
- W2359297762 hasConcept C92087593 @default.
- W2359297762 hasConceptScore W2359297762C103878061 @default.
- W2359297762 hasConceptScore W2359297762C104317684 @default.
- W2359297762 hasConceptScore W2359297762C145640855 @default.
- W2359297762 hasConceptScore W2359297762C153911025 @default.
- W2359297762 hasConceptScore W2359297762C159047783 @default.
- W2359297762 hasConceptScore W2359297762C167625842 @default.
- W2359297762 hasConceptScore W2359297762C202878990 @default.
- W2359297762 hasConceptScore W2359297762C22744801 @default.
- W2359297762 hasConceptScore W2359297762C2522874641 @default.
- W2359297762 hasConceptScore W2359297762C2777773057 @default.
- W2359297762 hasConceptScore W2359297762C2780530800 @default.
- W2359297762 hasConceptScore W2359297762C2781045504 @default.
- W2359297762 hasConceptScore W2359297762C33161422 @default.
- W2359297762 hasConceptScore W2359297762C40767141 @default.
- W2359297762 hasConceptScore W2359297762C47289529 @default.
- W2359297762 hasConceptScore W2359297762C54355233 @default.
- W2359297762 hasConceptScore W2359297762C86803240 @default.
- W2359297762 hasConceptScore W2359297762C92087593 @default.
- W2359297762 hasLocation W23592977621 @default.
- W2359297762 hasOpenAccess W2359297762 @default.
- W2359297762 hasPrimaryLocation W23592977621 @default.
- W2359297762 hasRelatedWork W2278116524 @default.
- W2359297762 hasRelatedWork W2354607804 @default.
- W2359297762 hasRelatedWork W2357443282 @default.
- W2359297762 hasRelatedWork W2357736261 @default.
- W2359297762 hasRelatedWork W2359561858 @default.
- W2359297762 hasRelatedWork W2363577614 @default.
- W2359297762 hasRelatedWork W2367411463 @default.
- W2359297762 hasRelatedWork W2368683852 @default.
- W2359297762 hasRelatedWork W2373332086 @default.
- W2359297762 hasRelatedWork W2373339635 @default.
- W2359297762 hasRelatedWork W2378274387 @default.
- W2359297762 hasRelatedWork W2381286482 @default.
- W2359297762 hasRelatedWork W2384885797 @default.
- W2359297762 hasRelatedWork W2385321428 @default.
- W2359297762 hasRelatedWork W2388204664 @default.
- W2359297762 hasRelatedWork W2390754526 @default.
- W2359297762 hasRelatedWork W2391319649 @default.
- W2359297762 hasRelatedWork W2413751509 @default.
- W2359297762 hasRelatedWork W2916412334 @default.
- W2359297762 hasRelatedWork W3143669357 @default.
- W2359297762 isParatext "false" @default.
- W2359297762 isRetracted "false" @default.
- W2359297762 magId "2359297762" @default.
- W2359297762 workType "article" @default.