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- W2359314057 abstract "The transformation and expression properties of a Bacillus thuringiensis plasmid free derivative strain BMB171 was reported. High transformation efficiencies in BMB171 were found as 1000 , 6.7 , 12.5 , 66.7 , 3500 , and were 2 folds as high as the highest values of 3 recipients Bt 4Q7, Bt 4D10 and Bti.IPS.78/11, respectively using 6 exogenous plasmids (pHT3101,pBMB3305,etc.) as the donor DNAs. The highest transformants could reach 10 7 per μ g DNA in BMB171. The stability of exogenous plasmids introduced in BMB171 was correlated with the patterns of replicons and sizes of plasmids. The properties of expressing cry 1Ac and cry 1Ca in BMB171 were also studied by quantifying the expression level and determinating the toxicity of the relevant transformants against two Lepidopteran larvae.It showed that the expression level of cry 1Ac gene in BMB171 was higher than that in Bt 4Q7, and less than those in Bt 4D10 and Bti.IPS.78/11 , but the expression level of cry 1Ca in BMB171 was higher than each of the three recipients; and the toxicity against Plutella xylostella produced by BMB171 transformants was higher than those by Bt 4Q7 and Bt 4D10, but less than that by Bti.IPS.78/11, whereas the toxicity against Spodoptera exigua larvae was higher than those produced by the three recipients." @default.
- W2359314057 created "2016-06-24" @default.
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- W2359314057 date "1999-01-01" @default.
- W2359314057 modified "2023-09-25" @default.
- W2359314057 title "TRANSFORMATION AND EXPRESSION PROPERTIES OF A BACILLUS THURINGIENSIS PLASMID FREE DERIVATIVE STRAIN BMB171" @default.
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