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- W2359319026 abstract "Objective To investigate the bone related gene expression in human osteoblasts cultured in vitro. Methods Human osteoblasts were isolated and cultured by the method of tissue fragment migrating growth.Their biological characteristics were observed alive with alkaline phosphatase staining.By the method of RT PCR,the human osteoblasts of second passage cultured for 14 days were examined for the expression of genes COL1,BGP,OPG,ON,IGF 1,and TGF β mRNA.Their expression was normalized relative to their housekeeping gene products GAPDH by the establishment of a gene/GAPDH ratio.Results Phase contrast inverted microscopy shows that the osteoblasts are triangle,quadrangle or irregular in shape.50%-90% of osteoblasts show positive alkaline phosphatase.The genes COL1,BGP,OPG,ON,IGF 1,and TGF β mRNA expression was observed in the human osteoblasts of second passage cultured for 14 days.Conclusion Human osteoblasts cultured in vitro can express COL1,BGP,OPG,ON,IGF 1 and TGF β mRNA.;" @default.
- W2359319026 created "2016-06-24" @default.
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- W2359319026 date "2002-01-01" @default.
- W2359319026 modified "2023-09-23" @default.
- W2359319026 title "Bone related gene expression in osteoblasts cultured in vitro" @default.
- W2359319026 hasPublicationYear "2002" @default.
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