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- W2359335094 abstract "AIM:To explore the production and cytotoxicity of the reactive oxygen species(ROS)induced by diallyl trisulfide(DATS)in HL-60 cells.METHODS:HL-60 cells were either treated with various doses of DATS alone,or DATS combination with apocynin,a specific NADPH oxidase inhibitor,or with antioxidant N-acetyl-L-cysteine(NAC)for 0,1,3,6,12 and 24 h,respectively.The intracellular ROS level was measured by flow cytometry.The activity of NADPH oxidase was evaluated by NBT reduction experiment.The content of both malondialdehyde(MDA)and the protein carbonyl were analyzed by spectrophotometer.RESULTS:The results from flow cytometry indicated that DATS significantly increased the intracellular ROS level in HL-60 cells(P0.05),which was dose-and time-dependent.The fluorescence intensities of ROS reached at maximum when HL-60 cells were incubated with 150 μmol/L DATS for 3 h.The NBT reduction experiment showed that DATS activated NADPH oxidase,the highest activity was observed when the cells were exposed to 150 μmol/L DATS for 3 h.DATS induced MDA and protein carbonyl production in HL-60 cells.Furthermore,both MDA and protein carbonyl reached the highest level in the cells exposed to 150 μmol/L DATS for 3 h.Apocynin and NAC attenuated the production of MDA and protein carbonyl,suggesting that ROS induced by DATS was involved in the toxicity to the cells.CONCLUSION:DATS induces ROS production through activating NADPH oxidase in HL-60 cells.ROS increases the oxidation of membrane lipid and protein in HL-60 cells." @default.
- W2359335094 created "2016-06-24" @default.
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- W2359335094 date "2008-01-01" @default.
- W2359335094 modified "2023-09-23" @default.
- W2359335094 title "Production and cytotoxicity of the reactive oxygen species induced by diallyl trisulfide in human myeloid leukemia HL-60 cells" @default.
- W2359335094 hasPublicationYear "2008" @default.
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