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- W2359335317 abstract "Cathaya argyrophylla Chun Kuang,a monotypic genus of the pine family,is endemic to China.Chinese government lists it as a Class-I protected plant because of its limited individuals in the wild.Natural regeneration is rare and it is imperative to better understand its eco-physiological characteristics of seed germination.Cones of Cathaya argyrophlla were collected from selected healthy and 60-100 years old individuals and there are 490 to 520 cones per kilogram.Every 100 cones produced 4.36 seeds and every 1 000 seeds weight 15.8 grams.TTC test concluded that the seed viability is 50.50%.Storage method and duration significantly affected seed germination.The highest germination rate,33.00%,was produced under moisture moss at 4 ℃ after storing for 30 days.Both moisture newspaper and sand did not greatly reduce germination rate at 30 days.However,average seed germination was significantly lower from 31.0% to 18.0% to 7.0% as the storage durations went from 30 to 60 to 90 days.After storing for 90 days,seed germination were 13.0% and 2.7% for storing in moisture moss and newspaper,respectively.For the long-term storage,moisture moss is recommended.Seed POD,SOD activities and MAD levels during storages changed dramatically.The highest POD and SOD activities,5.58 mmol/g and 300 U/(g min),occurred under moisture newspaper at 30 days,which significantly higher than other two storage methods.As storage days went up,no significantly difference occurred among three storage methods.The average POD and SOD were 0.48 and 100 at 60 days,0.71 and 96.8 at 90 days.MAD content kept high at 2.71 mmol/g under the moisture newspaper stored for 30 days,then from 0.08 to 0.51 for other storage methods and duration.After storing for 60 and 90 days,POD,SOD activities and MAD level were relatively lower and no significant difference was found among the storage methods.Hormones GA,NAA,and IAA could significantly influenced germination rates.Under the moisture newspaper storage,seeds soaked for 24 hours in 100 mg/L of NAA and IAA after storing for 30 days yielded 55.00% and 55.01% germination rates,which were 83.31% higher than that of the control at 30.0%.After 60 days,only NAA at 150 mg/L soak produced the significantly higher germination rate of 24.2% than that of the control at 16.0%.All other treatments reduced the germination rate significantly.After 90 days,NAA at 150 mg/L soak yielded 11.08% germination rate,which is significantly higher than that of the control at 4.62%.GA soaks under all three storage durations resulted significantly lower germination rates than that of the controls and should be avoided.For seeds stored at moisture sand and moss,GA had the similar effects as NAA and IAA.Regardless of storage durations and hormone concentrations,all three hormones had less significant impact on germination rates comparing with seeds stored in the moisture sand.NAA at 100 mg/L soaked for 24 hours is recommended for Cathaya argyrophylla seed germination.Lower or higher concentrations of NAA and IAA might promote seed germination,but not as well as 100 mg/L in general.As storage time went longer,NAA had better effect than that of IAA." @default.
- W2359335317 created "2016-06-24" @default.
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- W2359335317 date "2010-01-01" @default.
- W2359335317 modified "2023-09-26" @default.
- W2359335317 title "Seed germination of endangered Cathaya argyrophylla Chun & Kuang" @default.
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