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- W2359404493 abstract "The animo acid sequence of Drosophila eIF5A was searched against the silkworm EST database with tBLASTn program.The ESTs with high score were clustered and assembled to a consensus sequence.We cloned and indentified a gene in Bombyx mori using the primers based on the consensus sequence from assembly,termed it as BmeIF 5A-1(GeneBank accession number: DQ202521).We also cloned and identified the gene in silkworm using the protocol of 3′rapid amplification of cDNA ends(3′RACE),termed it as BmeIF 5A2(GeneBank accession number: DQ201182).It had the same ORF as the BmeIF 5A1,but a poly(A) tail.These results of the structure of the BmeIF 5A-1 and BmeIF 5A-2 indicated that BmeIF 5A gene is likely to contain two transcriptional types and different length of 3′untranslated region.The BmeIF 5A gene was abundant in silkgland tissue in the silkworm.An alignment of the coding sequence with the silkworm genome sequences revealed that there were 3 exons in BmeIF 5A.The deduced amino acid sequence of the BmeIF 5A shared a conservative domain harbored in a 12 amino acid sequence(STSKTGKHGHAK).It showed that the silkworm elF5A protein was conserved in almost eukaryotes comparing with other species.The phylogenetic analysis showed a higher conservation of the silkworm BmeIF 5A with the Arthropoda." @default.
- W2359404493 created "2016-06-24" @default.
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- W2359404493 date "2006-01-01" @default.
- W2359404493 modified "2023-09-23" @default.
- W2359404493 title "Cloning and Phylogenetic Analysis of Eukaryotic Initiation Factor 5A,an Abundant Gene in Tissue of Silkgland in Silkworm,Bombyx mori" @default.
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