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- W2359429376 abstract "Background and purpose:Carcinoma of the kidney is the most common malignant renal parenchymal carcinoma;its biological behavior is extremely complicated and is not sensitive to radiotherapy and chemotherapy.Studies have shown that the hypoxia-inducible factor HIF1-α and HIF2-α are related to the occurrence and development process of the clear-cell carcinoma of kidney.So,we intend to construct psilencer3.0-HIF-α siRNA recombinant plasmid in this study through RNA interference method,thereby providing an effective tool for further exploration of the role of HIF in the occurrence and development of clear-cell carcinoma of kidney.Methods:Design and chemically synthesize the DNA fragments of coding HIF-1α and HIF-2α siRNA and make them up into siRNA expression vector through gene recombination.Adopting the real-time quantitative PCR and Western blot tested the inhibitive effect of the constructed siRNA expression vector on mRNA and protein levels on the target gene expression.Results:After 786-0 cells transfecting with psilencer3.0-HIF-1αsiRNA and psilencer3.0-HIF-2α,the inhibitory rate of mRNA expression of HIF-1α and HIF-2α reached 82.1% and 87.4% respectively,and OS-RC-2 cells transfecting psilencer3.0-HIF-1α siRNA and psilencer3.0-HIF-2α,the inhibition rate reached 91.2% and 81.2% respectively.The protein expression of the experimental group with 786-0 cells and OS-RC-2 cells transfecting HIF-1α interference plasmid and HIF-2α interference plasmid were lower at different levels than that from the blank control group.Conclusions:The constructed siRNA expression vector can effectively inhibit the expression of target gene HIF-1 and HIF-2 at mRNA and protein levels." @default.
- W2359429376 created "2016-06-24" @default.
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- W2359429376 date "2007-01-01" @default.
- W2359429376 modified "2023-09-26" @default.
- W2359429376 title "Construction and identification of psilencer3.0-HIF-α siRNA" @default.
- W2359429376 hasPublicationYear "2007" @default.
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