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- W2359432860 abstract "Objective To test HBV DNA by using PCR-microfluidic chip assay. Methods Pooled sera ( 5×50ul ) negative for ELISA serological tests were tested for HBV DNA using PCR-microfluidic chips assay. Individual donor samples were tested if the pooled sera were positive. The sensitivity of PCR-microfluidic chips assay was determined by serial dilutions of the standard control serum. The specificity of PCR-microfludic chips assay was also determined by testing 56 various serum samples. Serial dilutions of the standard control sera were tested repeatedly for understanding the inter- and intra-assay variation of this method. Results Seven of 545 nonrenumerated donors (1.28%) were found positive for HBV DNA. The sensitivity of PCR-microfluidic chips assay was 4.81×102copies/ml. The HBV DNA was positive for all 37 samples from HBeAg positive patients. The HBV DNA tests of samples from HCV RNA positive patients, anti-HAV IgM positive patients were all negative. The inter- and intra assay CV ranges were 15.6%~40.2% and 11.9%~30.6% respectively. Conclusion It is necessary to test HBV DNA for improving blood safety and it is feasible to test pooled serum samples for HBV DNA by PCR-microfluidic chips assay, because it is convenient, time-saving, sensitive, specific and the results are reproducible." @default.
- W2359432860 created "2016-06-24" @default.
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- W2359432860 date "2005-01-01" @default.
- W2359432860 modified "2023-09-24" @default.
- W2359432860 title "HBV DNA assay using PCR-microfluidic chips for blood screening" @default.
- W2359432860 hasPublicationYear "2005" @default.
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