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- W2359456031 abstract "For detection receptor of porcine reproductive and respiratory syndrome virus(PRRSV) in permissive cells,a real time TaqMan RT-PCR was developed using specific primers and fluorogenetic probe based on the gene of Sn and CD163 receptor of PRRSV.The established method had a linear dynamic range from 101 copies/μL to 108 copies/μL.The result demonstrated that the real-time RT-PCR assay was highly specific and sensitive with a detection limit of 10 copies for both Sn and CD163.The coefficient of variation for both intra-and inter-assay was less than 5%.The assay was used to detect Sn and CD163 mRNA transcription in porcine alveolar macrophage(PAM) cells at 72 hours post infected with PRRSV.The result showed the transcription levels of Sn and CD163 mRNA were both up-regulated significantly.The assay demonstrated that the real-time RT-PCR assay was highly specific and sensitive." @default.
- W2359456031 created "2016-06-24" @default.
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- W2359456031 date "2012-01-01" @default.
- W2359456031 modified "2023-09-26" @default.
- W2359456031 title "Establishment of TaqMan fluorescence quantitative RT-PCR assay for PRRSV Sn and CD163 receptor detection" @default.
- W2359456031 hasPublicationYear "2012" @default.
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