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- W2359467507 abstract "【Objective】To assay the promoting-maturation activity of MPF to induce immature oocytes to mature in vitro and to observed the metaphase chromosomes of the single mature oocytes.【Methods】Hela cells were blocked at mitotic metaphase by colcemid in order to extract the proteins containing MPF.The culture medium was individed into 3 groups,non-hormone group(without hormone),hormone group(with hormone),injecting-group(without hormone).MPF extracts were microinjectied into immature oocytes of Kunming mouse in the injecting-group medium.Observation and statistic analysis of the cell number with GVBDs(germinal vesicle breakdown) or extrued polar bodies were made among the three group.The metaphase chrosomes of mature oocytes were prepared and observed.【Results】The significant difference of the percent of GVBDs was demonstrated between non-hormone group and injectied-group by statistics(P0.05).The percentage of extrued polar bodies in injected group was significantly higher than non-hormone group and hormone group(53.4% vs 31.08%,33.3%),The significant difference was demonstrated between the injectied-group and non-hormone group,or hormone group by statistics(P0.001,P0.005).And the metaphase chromosomes of the single mature oocytes were observed.【Conclusion】The results indicated MPF extract possess the property that can induce immature oocytes to reinitiate meiosis." @default.
- W2359467507 created "2016-06-24" @default.
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- W2359467507 date "2008-01-01" @default.
- W2359467507 modified "2023-09-25" @default.
- W2359467507 title "Assay of promoting-maturation activity of M-phase promoting factor(MPF) from Hela cell" @default.
- W2359467507 hasPublicationYear "2008" @default.
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