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- W2359494069 abstract "Objective: To investigate the expression of Smo gene in human hepatocellular carcinoma Huh-7 cells in cytological level and the impact of mRNA interference with Smo gene expression on hepatoma Huh-7 cells proliferation and apoptosis.Methods: Huh-7 cell culture,total RNA extraction,UV spectrophotometer determination of purity,Western imprinting method were used for the detection of Smo protein expression.After transfection,flow cytometry was applied to detect the Huh-7 apoptosis rate.Results: Smo were strongly expressed in mRNA and protein levels.SiRNA-1 made the strongest interference results,and could induce apoptosis of Huh-7 cells after transfection.Conclusion: siRNA-l had interference effects on Smo gene expressionin liver cancer Huh7 cells.siRNA-1 sequence can effectively degrade Smo mRNA in hepatoma Huh7 cells,down-regulate theSmo mRNA and Smo protein expression,so that can silence the Smo mRNA expression of hepatoma Huh7 cells." @default.
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- W2359494069 date "2012-01-01" @default.
- W2359494069 modified "2023-09-24" @default.
- W2359494069 title "Expression of Smo gene in human hepatoma Huh-7 cells and effect of mRNA interference with Smo gene expression on proliferation and apoptosis of hepatoma Huh-7 cells." @default.
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