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- W2359497777 abstract "Objective To investigate the effect of external counterpulsation(EECP)-conditioned serum on gene expression of vascular endothelial cells by cDNA microarray. Methods EECP-conditioned sera were collected at 1 h , 24 h and 36 h time points of EECP therapy. Serum of 1 h post-EECP was used as EECP treatment serum, and of pre-EECP as control. After human umbilical vein endothelial cells(HUVEC) were cultured with above EECP conditioned-sera of different time points, gene expression from HUVEC was screened with cDNA microarray respectively. 1 h point EECP treatment effect was compared with 24 h,36 h points.Results Ten genes were up-regulated(by more than 2-fold) in 1 h point, and down-regulated in 24 h, 36 h points (by more than 1- or 2-fold), including NF-κB, eukaryotic translation initiation factor 4 gamma 1, myosin heavy polypeptide smooth muscle, actinin alpha 2, tubulin beta polypeptide, HLA-G, melanoma adhesion molecule, neuromedin B receptor, MAP4K 2, thrombospondin 1. Conclusions Changes of gene expression induced by EECP-conditioned serum depend on different treatment times. EECP-conditioned serum can reduce inflammatory reaction and apoptosis in endothelial cells which results in the amelioration of atherosclosis." @default.
- W2359497777 created "2016-06-24" @default.
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- W2359497777 date "2006-01-01" @default.
- W2359497777 modified "2023-09-28" @default.
- W2359497777 title "Effect of enhanced external counterpulsation treatment serum on the gene expression of vascular endothelial cell" @default.
- W2359497777 hasPublicationYear "2006" @default.
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