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- W2359500228 abstract "Aim To investigate whether apoptosis of HeLa cells can be induced by recombinant Caspase 6(RevCasp6), constructed from reversing the two subunits of Caspase 6 . Methods Human Caspase 6 gene was obtained through RT PCR, and was neformed into recombinant Caspase 6 by settling the small subunit prior to the large one through PCR. Both genes of Caspase 6 and recombinant Caspase 6 were proved without mutation by sequencing. RevCasp6 gene was inserted into the vector pIRSE2 EGFP.With pIRES2 EGFP as negative control,the gene was transfected into HeLa cells. Green fluorescence and cell condition were observed under the fluorescentce microscopy and electron microscopy.Results The cells transfected with recombinant caspase 6 are in a very bad condition, and the fluorescentce can be seen in the nucleus. And the morphological features were in accordance with that of apoptotic cells. Conclusion Reconstructed caspase 6 gene can kill the cells mainly through inducing apoptosis." @default.
- W2359500228 created "2016-06-24" @default.
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- W2359500228 date "2002-01-01" @default.
- W2359500228 modified "2023-09-25" @default.
- W2359500228 title "Investigation of apoptosis of the HeLa cells induced by reconstructed Caspase 6" @default.
- W2359500228 hasPublicationYear "2002" @default.
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