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- W2359536744 abstract "【Objective】The objective of the study is to clone the complete cDNA sequence of SFB and probe into polymorphism in the Central Asian ecotype group apricot.【Method】2 Apricot(Prunus armeniaca) cultivars ‘Mantouyuluke’ and ‘Suogejianali’ belonging to the Central Asian ecotype group were used as experimental materials and Two complete cDNAs of SFB gene(SFB33 and SFB 50) were cloned by RTPCR and Rapid Amplification of cDNA Ends(RACE) techniques,and analyzed with DNAMAN software.【Result】The results showed that Two complete cDNAs of SFB gene(SFB33 and SFB 50) were cloned.SFB33(HQ148064) was 1 378 bp and SFB50(HQ148074) was 1 313 bp in full length,the two genes were a ORF for 1 131 bp,encoding 376 amino acids.Compared with the nucleotide sequences registered in GenBank,the highest homology of SFB33 was 79% with SFB-c(FJ362527) and SFB50 was 99% with SFB23(EU652886).F-box regions,two variable regions and two hypervariable regions were both found in the two genes.Based on the deduced amino acid sequence alignment in Prunoideae,SFB 50 was closer with other SFB gene,but SFB50 was far from them in Homology tree.【Conclusion】The studies suggested that there were polymorphism in sequences of SFB gene from the Central Asian ecotype group apricot." @default.
- W2359536744 created "2016-06-24" @default.
- W2359536744 creator A5003062954 @default.
- W2359536744 date "2013-01-01" @default.
- W2359536744 modified "2023-09-26" @default.
- W2359536744 title "Cloning and sequence analysis of 2 SFB genes in apricot cultivars belonging to the Central Asian ecotype group" @default.
- W2359536744 hasPublicationYear "2013" @default.
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