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- W2359549395 abstract "Two RNases (Ⅰ and Ⅱ) were isolated from extracts of malted barley roots by ammonium sulfate precipitation and purified by chromatography on Sephacryl S 200, DEAE SephadexA 50 and Sephacryl S 200 consecutively. The RNase Ⅰ was purified by 94 fold and RNase Ⅱ 1790 fold. Reaction products of these two RNases with yeast RNA were all 5′ nucleotides determined by HPLC. The heat stability of RNase Ⅰ was better than that of RNase Ⅱ. Activity loss was 64% for RNase Ⅱ and less than 13% for RNase Ⅰ after 2 h at 55 ℃. The optimum temperatures of RNase Ⅰ and RNase Ⅱ were 65 ℃ and 55 ℃ respectively. The pH stability of these two enzymes was similar and they were stable in the pH 2.5～9.0. The optimum pHs were 5.0 and 8.0～8.5 for RNase Ⅰ, and pH 4.0～5.5 for RNase Ⅱ." @default.
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- W2359549395 date "2001-01-01" @default.
- W2359549395 modified "2023-09-25" @default.
- W2359549395 title "Separation, Purification and Properties of RNase in Malted Barley Roots" @default.
- W2359549395 hasPublicationYear "2001" @default.
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