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- W2359554048 abstract "Objective: To improve the cultivating method of retinal pigment epithelium cells (RPE) in rabbits. Methods:The eye ball was bisected along the equator on a sterile filter paper. The anterior portion of eye was discarded, then the posterior eye cup was fixed in a rubber bysma. The single cell was collected directly by trypsinization. The first time for the medium to be changed was prolonged from 48 h to 72 h. The RPE cells were identified by DOPA staining and immunohistochemistry. Results: RPE cells grew in well-distributed way rather than in colony in this experimental condition. Prolonging first changing medium time was found to increase the number of cells. RPE cells reached confluence more quickly and the melanin disappeared more slowly. Conclusion: The modified method is more suitable for cultivating RPE cells in rabbits." @default.
- W2359554048 created "2016-06-24" @default.
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- W2359554048 date "2003-01-01" @default.
- W2359554048 modified "2023-09-24" @default.
- W2359554048 title "Improvement of Culture of Retinal Pigment Epithelium Cells in Rabbits" @default.
- W2359554048 hasPublicationYear "2003" @default.
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