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- W2359579569 abstract "Aim: To investigate the best condition of survivin antisense oligodeoxynucleotides(ASODN) mediated by polyethyleneimine on the proliferatory of hepatocelluar carcinoma SMMC-7721 cells.Methods: Gel electrophoresis retardation assays sieve the best qualitative ratio of PEI /ASODN.Cytotoxic effects of PEI were quantified by WST-8 test in vitro.The instantaneous and 48 h transfection efficiencies of PEI were determined by flow cytometry and WST-8 test in various ratios of PEI / ASODN.Similarly,the uptake rates of FAM labeled PEI-ASODN or ASODN on SMMC-7721 cells were tested by flow cytometry,while the morphological uptaking of FAM labeled PEI-ASODN or ASODN was observed under fluorescence microscope.The influence of bovine serum culture on PEI transfection efficiency was detected by WST-8 test.Results: PEI could retard ASODN migration when PEI/ASODN ratio varied from 0.625∶1 to 2.5∶1.When incubated with PEI for 48 hours,the 85 % survival rate of SMMC-7721 cells appeared at 4 μg/mL,and when the PEI/ASODN ratio is 0.75 μg/mL,the best transfection efficiency of PEI appeared.The bovine serum in the culture medium didn't influence the transfection efficiency.Conclusion: It is certificated that PEI is low-toxicant as ASODN transfection vector,and can be used to transfer ASODN to hepatocelluar carcinoma when it is under 4 μg/mL.The best transfection efficiency of PEI-ASODN on hepatocelluar carcinoma SMMC-7721 cells is tested when the PEI/ASODN ratio is 0.75 μg/mL with the bovine serum culture medium in vitro." @default.
- W2359579569 created "2016-06-24" @default.
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- W2359579569 date "2006-01-01" @default.
- W2359579569 modified "2023-09-25" @default.
- W2359579569 title "Optimization of conditions of survivin antisense oligodeoxynucleotides mediated by polyethyleneimine on hepatocelluar carcinoma SMMC-7721 cells in vitro" @default.
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