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- W2359631708 abstract "The method of cross-linking-entrapment is adopted to immobilize β-glucosidase in sodium alginate solution on the basis of selection of eight immobilization carriers.And the properties of the immobilized enzyme are researched.The results show that the recovery percentage of enzyme activity was 83.67% when the immobilized enzyme was prepared by cross-linking for two hours using 0.2% glutaraldehyde,subsequent mixing with 2.0% sodium alginate solution,then adding the obtained mixture dropwise into the 0.40% CaCl2 solution and solidificating for one hour.The properties of the immobilized β-glucosidase were superior to the free enzyme,including the optimal temperature,thermal and pH stability and affinity for the substrate.The immobilized β-glucosidase was employed to produce genistein,and 90.94% of the activity and 60.02% of conversion were kept as the enzyme was consecutively used for six times." @default.
- W2359631708 created "2016-06-24" @default.
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- W2359631708 date "2007-01-01" @default.
- W2359631708 modified "2023-09-24" @default.
- W2359631708 title "Preparation and properties of sodium alginate-immobilized β-glucosidase" @default.
- W2359631708 hasPublicationYear "2007" @default.
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