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- W2359637478 abstract "Objective: To increase efficiency in expression of apoptin gene(vp3) in tumor cells and promote its bioactivity to induce apoptosis of tumor cells. Methods: The plasmid pcDNA-VP3 was used as a template and the apoptin gene with Kozak sequence,i,e.eukaryn ribosome locus was amplified through twice PCR method. The recombined apoptin gene was subcloned into the multiple clone site of plasmid pRevTRE to obtain a plasmid pRevTRE-VP3. The plasmid pRevTRE-VP3 was cut and identified by means of restriction endonuclease BamHI and XhoI. The sequence of selected plasmid pRevTRE-VP3 was analysed. Results: The sequence of recombined apoptin gene was identical with that reported by Noteborn et al. The apoptin gene in which Kozak sequence was added to the front of ATG code was subcloned into the eukaryn vector pRevTRE. Conclussion: The eukaryn ribosome locus which promotes expression efficiency may be added to the front of ATG code of sequence of apoptin gene by twice PCR and the eukaryn vector for expression of apoptin can be constructed." @default.
- W2359637478 created "2016-06-24" @default.
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- W2359637478 date "2005-01-01" @default.
- W2359637478 modified "2023-09-28" @default.
- W2359637478 title "Study on recombination of sequence of apoptin gene and construction of a eukaryn vector for expression of apopt" @default.
- W2359637478 hasPublicationYear "2005" @default.
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