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- W2359652162 abstract "In order to construct the DNA vaccine, the gene TA4 was inserted into expression vector pcDNA3.1(Zeo~+), and then the gene Et1A was ligated to its upper stream, aiming to express them in a fusion protein form. The IFAT was established to examine the in vitro expression of the constructed pcDNA-TA4 (pCT) and pcDNA-Et1A-TA4 (pCTE) in the COS cell, and positive results were gained. In the clinical experiment, the DNA vaccines were given to the chickens i.m. 10～15 min after the breast injection was pre-treated with 25% sucrose solution. After being vaccinated twice at 1~(st) and 2~(nd) wk of age, chickens were challenged with 3×10~4 sporulated oocysts of E. tenella at the 3~(rd) week of age. At the 8~(th) day post inoculation, chickens were killed and the lesion scores of caeca , the relative body weight gains, the numbers of oocysts in the caeca of each group were calculated and compared. The anti-coccidial index (ACI) was used as a standard to compare the effects of each treatment. Results indicated that, both the pCTE and pCT had definitive protection against challenge, they could obviously attenuate the body weight decrease following coccidial inoculation. When 50 μg pCT was given to the chickens along with the IFN-γ, chickens had the maximum body weight gains (108.8% of that of the unchallenged control group) and the minimum lesion scores in all the inoculated groups, its ACI was 167.2. While the ACI of 160 was achieved with 50 μg pCTE compared with 100 μg pCT. This means that the fusion expression of two protective antigen genes in DNA vaccine can spare the use of the constructed plasmid, and so decrease the cost of DNA vaccine in the long run." @default.
- W2359652162 created "2016-06-24" @default.
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- W2359652162 date "2004-01-01" @default.
- W2359652162 modified "2023-09-25" @default.
- W2359652162 title "Construction of the DNA vaccine pCT and pCTE and their efficacy against experimental coccidiosis in chickens" @default.
- W2359652162 hasPublicationYear "2004" @default.
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