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- W2359659812 abstract "Objective To investigate a new method for detection and purification of ScFv from culture supernatants. Methods Relative sensitivity of various conjugates in ELISA for the detection of ScFv was assessed. The culture supernatants containing ScFv was ultrafiltered, and then precipitated with 313 g/L saturated ammonium sulfate. ScFv were finally purified by protein L-agarose affinity column. Results The maximum titer with positive result was 1∶16 detected by HRP-protein L, while the maximum titer with positive result was only 1∶2 by HRP-protein A; and the 9E10 antibody gave negative results except poor sensitivity was observed in non-diluted culture supernatants well. The purified ScFv showed a high purity and good activities as monitored by SDS-PAGE and ELISA. Conclusion Protein L is a very sensitive and effective Ig-binding protein to detect and purify ScFv.The described method is a simple and highly effective one." @default.
- W2359659812 created "2016-06-24" @default.
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- W2359659812 date "2004-01-01" @default.
- W2359659812 modified "2023-09-25" @default.
- W2359659812 title "Detection and purification of ScFv from culture supernatants by protein L" @default.
- W2359659812 hasPublicationYear "2004" @default.
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