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- W2359693039 abstract "Objective To investigate the absorption characteristics of HMME and inhibitory effect of HMME-mediated photodynamic therapy on human keratinocyte. Methods Exponentially growing keratinocyte cell line Hacat was incubated with a serial doses of HMME(0-40μg/ml) for 1h or incubated with 10μg/ml HMME for various time(0-120min).Both of the HMME absorption were measured by FACS.To test the inhibitory effect of HMME-PDT, Hacat cell was incubated with HMME(2.5-40μg/ml) for 2h, then exposure to frequency-doubled Nd∶YAG laser(532nm, 20mW/cm~2)for 0, 2.5, 5, 10, 20min, respectively.Cell viability was assessed by MTT assay 24 h after PDT. Results The absorption of HMME by Hacat cells was increased with incubation concentrations and time.Light alone or HMME alone had no effect on viability of Hacat cell.The inhibitory effect of PDT was in proportion to the HMME concentration and light dose. Conclusions The absorption of HMME by Hacat cells is dose-dependent and time-dependent, and HMME-PDT have significant inhibitory effect on Hacat cells, the concentration of incubation and energy density are two important factors affecting the inhibitory effect of HMME-PDT." @default.
- W2359693039 created "2016-06-24" @default.
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- W2359693039 date "2006-01-01" @default.
- W2359693039 modified "2023-09-25" @default.
- W2359693039 title "Inhibitory Effect of HMME-mediated Photodynamic Therapy on Proliferation of Human Keratinocyte in vitro" @default.
- W2359693039 hasPublicationYear "2006" @default.
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