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- W2359694294 abstract "A sensitive and specific liquid chromatography electrospray ionization ion-trap mass spectrometry(LC-ESI MS/MS) method has been developed for the determination of biotin in infant formula food.After pretreatment procedure,sample was separated on a Dikma C18(4.6 mm×250 mm,5 μm) column with a mobile phase consisting of methanol(0.5% formic acid,by volume)-20 mmol/L ammonium acetate(0.5% formic acid)(40∶60,by volume),and injection volume of 80 μL at a flow-rate of 1.0 mL/min.The split ratio of post column was 1∶3(by volume) and the column temperature was 25 ℃.Detection was performed by Agilent MSD Trap XCT ion-trap mass spectrometer using multiple reaction monitoring(MRM) mode via electrospray ionization(ESI) source and selecting m/z 245.3→226.9 as detecting ion.A good linear calibration was obtained for 2.50-100.0 μg·L-1 biotin with a correlation coefficient of 0.999 5.The spiked recoveries were in the range of 87%-101%(n=5).The detection limit of biotin concentration was 0.35 μg·L-1 and the detection limit of method was 0.7 ng/g.The proposed method is simple,sensitive and reproducible enough to be used for the determination of biotin in infant formula food." @default.
- W2359694294 created "2016-06-24" @default.
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- W2359694294 date "2009-01-01" @default.
- W2359694294 modified "2023-09-28" @default.
- W2359694294 title "Determination of Biotin in Infant Formula Food by High-performance Liquid Chromatography Coupled with Electrospray Ionization Mass Spectrometry" @default.
- W2359694294 hasPublicationYear "2009" @default.
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