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- W2359694520 abstract "Treatment of the seedlings in the 4℃ refrigerator, MES and preconditioning conditions were investigated for explants browning of winter type Brassica napus. Browning frequency of hypocotyls decreased significantly when the seedlings were treated in the 4℃ refrigerator for 12 hours, or the hypocotyls explants were cultured in the media containing MES. Further extension of the treatment period, however, resulted in higher browning frequency. As to the cotyledons, the browning frequency was decreased significantly when the cotyledons were preconditioned in MSB5 medium containing 1mg/L BA and 1mg/L 2,4-D for three days before 3-day incubation with Agrobacterium tumefaciens strain GV3101 harboring the binary plasmid pBI121." @default.
- W2359694520 created "2016-06-24" @default.
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- W2359694520 date "2004-01-01" @default.
- W2359694520 modified "2023-09-23" @default.
- W2359694520 title "Effect of preconditioning on explants browning of genetic transformation in winter type Brassica napus" @default.
- W2359694520 hasPublicationYear "2004" @default.
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