Matches in SemOpenAlex for { <https://semopenalex.org/work/W2359726167> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2359726167 abstract "Objective The pEGFP-N1-H2B1 plasmid vector was transfected to mouse vascular endothelial cell(VEC)to investigate the effects of H2-Bl gene on the apoptosis and proliferation,and then speculated the possible mechanism of immunological rejection after heart transplantation mirroring maternal-fetal immune tolerance.Methods The mouse VEC was transfected by 0.5 μg/ml of pEGFP-N1 plasmid vector,0.5 μg/ml of pEGFP-N1-H2B1 plasmid vector and 1.0 μg/ml of pEGFP-N1-H2B1 plasmid vector,respectively.The efficiency of transfection was detected;the H2-Bl mRNA level,the apoptosis and proliferation of VEC were also investigated at 24 h,48 h and 72 h,respectively.Results Expression of the H2-Bl gene was determined by real time quantitative PCR.Expression of the H2-Bl in 0.5 μg/ml and 1.0 μg/ml of pEGFP-N1-H2B1 plasmid vector groups was higher than that of the control group(P0.05,P0.001,respectively).VEC cell apoptosis was promoted after 48 h and 72 h in the 1.0 μg/ml H2-Bl gene group compared with control group(P0.05).VEC cell proliferation was inhibited in the H2-Bl gene groups at 24 h,48 h and 72 h,respectively(P0.01).Conclusions Transfection of pEGFP-N1-H2B1 plasmid vector to mouse VEC can increase the expression of H2-Bl mRNA,induce the apoptosis and inhibit proliferation,as a result,weaken the cytoactivity and induce immune tolerance." @default.
- W2359726167 created "2016-06-24" @default.
- W2359726167 creator A5006605854 @default.
- W2359726167 date "2010-01-01" @default.
- W2359726167 modified "2023-09-23" @default.
- W2359726167 title "Effects of H2-Bl gene transfection on the apoptosis and proliferation of mouse vascular endothelial cell" @default.
- W2359726167 hasPublicationYear "2010" @default.
- W2359726167 type Work @default.
- W2359726167 sameAs 2359726167 @default.
- W2359726167 citedByCount "0" @default.
- W2359726167 crossrefType "journal-article" @default.
- W2359726167 hasAuthorship W2359726167A5006605854 @default.
- W2359726167 hasConcept C104317684 @default.
- W2359726167 hasConcept C105580179 @default.
- W2359726167 hasConcept C111599444 @default.
- W2359726167 hasConcept C153911025 @default.
- W2359726167 hasConcept C190283241 @default.
- W2359726167 hasConcept C22744801 @default.
- W2359726167 hasConcept C33161422 @default.
- W2359726167 hasConcept C40767141 @default.
- W2359726167 hasConcept C54009773 @default.
- W2359726167 hasConcept C54355233 @default.
- W2359726167 hasConcept C62112901 @default.
- W2359726167 hasConcept C86803240 @default.
- W2359726167 hasConceptScore W2359726167C104317684 @default.
- W2359726167 hasConceptScore W2359726167C105580179 @default.
- W2359726167 hasConceptScore W2359726167C111599444 @default.
- W2359726167 hasConceptScore W2359726167C153911025 @default.
- W2359726167 hasConceptScore W2359726167C190283241 @default.
- W2359726167 hasConceptScore W2359726167C22744801 @default.
- W2359726167 hasConceptScore W2359726167C33161422 @default.
- W2359726167 hasConceptScore W2359726167C40767141 @default.
- W2359726167 hasConceptScore W2359726167C54009773 @default.
- W2359726167 hasConceptScore W2359726167C54355233 @default.
- W2359726167 hasConceptScore W2359726167C62112901 @default.
- W2359726167 hasConceptScore W2359726167C86803240 @default.
- W2359726167 hasLocation W23597261671 @default.
- W2359726167 hasOpenAccess W2359726167 @default.
- W2359726167 hasPrimaryLocation W23597261671 @default.
- W2359726167 hasRelatedWork W2347532513 @default.
- W2359726167 hasRelatedWork W2347877282 @default.
- W2359726167 hasRelatedWork W2348914301 @default.
- W2359726167 hasRelatedWork W2350212540 @default.
- W2359726167 hasRelatedWork W2354674969 @default.
- W2359726167 hasRelatedWork W2359500199 @default.
- W2359726167 hasRelatedWork W2360212884 @default.
- W2359726167 hasRelatedWork W2372918449 @default.
- W2359726167 hasRelatedWork W2376555927 @default.
- W2359726167 hasRelatedWork W2377022585 @default.
- W2359726167 hasRelatedWork W2377127973 @default.
- W2359726167 hasRelatedWork W2379715495 @default.
- W2359726167 hasRelatedWork W2383168431 @default.
- W2359726167 hasRelatedWork W2383787333 @default.
- W2359726167 hasRelatedWork W2390053359 @default.
- W2359726167 hasRelatedWork W2401083838 @default.
- W2359726167 hasRelatedWork W2473841395 @default.
- W2359726167 hasRelatedWork W3029301478 @default.
- W2359726167 hasRelatedWork W3030126837 @default.
- W2359726167 hasRelatedWork W3032272495 @default.
- W2359726167 isParatext "false" @default.
- W2359726167 isRetracted "false" @default.
- W2359726167 magId "2359726167" @default.
- W2359726167 workType "article" @default.