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- W2359751015 abstract "Objective A multiplex reverse transcription polymerase chain reaction (mRT-PCR) method is capable of detecting and subtyping the main respiratory viruses,including influenza A (H1N1 and H3N2) and B viruses as well as respiratory syncytial virus (RSV) types A and B.mRT-PCR method is establishd in order to detect the main viruses in respiratory clinical samples and the outbreak and epidemic of influenza in Shanghai.Methods Influenza A (H1N1,and H3N2),influenza B and RSV types A and B cultured by MDCK shell viral are adopted to detect their DNA zone spectrum in ultraviolet after mRT-PCR and agarose gel electrophoresis.Results Corresponding five obvious DNA zone spectrums at 431?bp,210?bp,390?bp,334?bp and 183?bp respectively in ultraviolet after mRT-PCR and agarose gel electrophoresis.Conclusions Using five pairs of primers,mRT-PCR could be used to detect influenza virus and to subtype the influenza A and RSV in clinical samples rapidly ,which is very important for diagnosis and surveillance of upper respiratory tract infectious diseases." @default.
- W2359751015 created "2016-06-24" @default.
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- W2359751015 date "2003-01-01" @default.
- W2359751015 modified "2023-09-27" @default.
- W2359751015 title "Main respiratory viruses detected by multiplex reverse transcription polymerase chain reaction" @default.
- W2359751015 hasPublicationYear "2003" @default.
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