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- W2359777771 abstract "Apoptin gene of the 46th passage chicken anaemia virus was amplyfied by polymerase chain reaction and cloned into pGEX_5X_3 vector.The recombinants were identified by restrictive enzyme digestion and sequencing.There are 363 base pairs(bp) in the apoptin gene which encode 121 amino acides.There is one base difference between the apoptin gene of the 46th passage and sequence of CAV Cux strain reported by Meehan B.M.et al at position 209,T C respectively.Compared the apoptin gene of the 46th Cux strain to CAV isolaes from Australia and America,there are 5 and 2 base differences respectively,which all located among the 210 bp region near the 5' terminer evenly.But the sequence is conserved near the 3' terminer.There is no difference between the apoptin gene of the 46th Cux strain and CAV isolates from the United Kindom.The hydrophilicity plot and the antigenic index of the apoptin encode by the positive clone were predicted in this study." @default.
- W2359777771 created "2016-06-24" @default.
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- W2359777771 date "2001-01-01" @default.
- W2359777771 modified "2023-09-25" @default.
- W2359777771 title "The Amplification,Cloning and Sequence Analysis of Apoptin Gene of the 46th Passage Chicken Anaemia Virus" @default.
- W2359777771 hasPublicationYear "2001" @default.
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