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- W2359781491 abstract "A serine-threonine specific protein phosphatase gene of Schistosoma japonicum,SjPP,was cloned into pET28a(+) to construct a recombinant plasmid pET28a(+)-SjPP.The plasmid pET28a(+)-SjPP in E.coli strain BL21(DE3) was induced with 0.1mmol/L of IPTG and expressed in inclusion bodies.Production of the recombinant protein rSjPP was 17.8mg per gram of bacteria after induction for 2h.The purified recombinant proteins were used to vaccinate BALB/c mice to evaluate immunoprotective efficacy.The mice in vaccination group were immunized with 20μg rSjPP emulsified with Freund′s complete adjuvant(FCA),and those in adjuvant or blank control groups were injected with FCA/FIA or PBS,respectively on week 0,2,4.The mice were challenged with cercariae on week 2 after the final injection,and were sacrificed on day 42 after challenge.High titre specific anti-rSjPP antibody IgG was observed in the sera of vaccinated mice,and partial worm reduction,hepatic tissue egg reduction,and significant faecal egg reduction were obtained in vaccinated group,when comparied with that in the blank control group." @default.
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- W2359781491 date "2006-01-01" @default.
- W2359781491 modified "2023-09-27" @default.
- W2359781491 title "Expression and protective immunity of serine-threonine specific protein phosphatase SjPP of Schistosoma japonicum" @default.
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