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- W2359790883 abstract "【Objective】 The objectives of this study are to investigate the occurrence of Potato virus Y(PVY) disease in China,to develop fast and accurate PVY detection methods and to understand sequence variations in PVY CP gene.【Method】ELISA method was used to detect the infected leaf samples randomly collected from 14 provinces(municipalities) in China during 2011 and 2012.A subsample of the infected leaves was examined using a transmission electron microscope.CP gene of PVY was amplified by reverse-transcription polymerase chain reaction(RT-PCR) using a pair of degenerate primers designed from the conserved regions of published CP gene sequences.Sequence variation was analyzed and phylogenetic tree was re-constructed using Bayesian inference method.【Result】ELISA revealed that 220 out of the 691 samples assayed were infected with PVY.The pin-wheel inclusion bodies were visualized clearly under the transmission electron microscope in the subsamples of ELISA-positive leaves.RT-PCR amplifications of the 14 ELISA-positive subsamples randomly selected from different provinces(municipalities) all generated an expected fragment of ≈800 bp in size.CP genes in the selected 14 subsamples shared more than 88% nucleotide identity with the reported sequences in other PVY strains.Among the 14 CP gene sequences in the subsamples,29 polymorphic sites were detected,including 6 parsimony informative and 23 singleton variable sites.Some of the sequence variations were geography-specific.Bayesian inference of phylogenetic trees revealed that 14 PVY isolates were in the same branch with reference PVYN:O strains,suggesting that the 14 PVY isolates analyzed in this study shared high homology with PVYN:Ostrain.【Conclusion】CP gene in PVY is highly conserved but some geographical variation in sequences exists.The results reported in this manuscript provide useful insights in understanding the evolution of PVY,the epidemiology and control of PVY disease in potato." @default.
- W2359790883 created "2016-06-24" @default.
- W2359790883 creator A5083618578 @default.
- W2359790883 date "2013-01-01" @default.
- W2359790883 modified "2023-09-23" @default.
- W2359790883 title "Detection and Molecular Variation of Potato virus Y CP Gene in China" @default.
- W2359790883 hasPublicationYear "2013" @default.
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