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- W2359792303 abstract "The optimal conditions of RAPD amplification of Emmenopterys henryi from Tiantai mountain in Zhejiang province was screened.After the analysis of the content of Mg~(2+),dNTP,DNA templates,primers and DNA polymerase,the optimal conditions of RAPD was determined as follows:1×Taq polymerase corresponding buffer,1.8 mmol·L~(-1) MgCl_2,2U Taq DNA polymerase,10 ng template DNA,20 pmol primer,0.1 mmol·L~(-1) dATP,dCTP,dGTP,dTTP for each in total 15μL reaction volume.12 random primers were selected in the amplification and 51 repetitive loci were produced.The percentage of polymorphic loci was 45.1% and the genetic diversity within population of E.henryi was relatively low,as indicated by Shannon index of phenotypic diversity and Nei index.It was significance for the further study on the genetic diversity of E.henryi from different populations." @default.
- W2359792303 created "2016-06-24" @default.
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- W2359792303 date "2004-01-01" @default.
- W2359792303 modified "2023-09-26" @default.
- W2359792303 title "Optimization of the RAPD Conditions of Emmenopterys henryi and Primary Study on the Genetic Diversity" @default.
- W2359792303 hasPublicationYear "2004" @default.
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